Skip to content

POD5 Outputs

POD5 is Oxford Nanopore Technologies raw read format, based on Apache Arrow. Its specification is part of the repository which implements the format. You can also find documentation for the format here.

Specification

The POD5 file format is documented here.

Paths

The following path patterns are used to place the data on disk.

File Path Path pattern
POD5 Default pod5{basecall_status_skip_or_empty}/{flow_cell_id}{basecall_status_skip_or_empty}_{short_protocol_run_id}_{short_run_id}_{batch_number}.pod5
Split by barcode Enabled pod5{basecall_status}/{alias}/{flow_cell_id}{basecall_status}_{alias_}{short_protocol_run_id}_{short_run_id}_{batch_number}.pod5

Note the pattern used is different when the option to split files by barcode is enabled.

See the Patterns documentation for more information on file patterns.

Read batching

The following batching options are used by default:

Option Value
Duration 3600s
Bases 500000000 Bases

For more information on batching see Batching.

Minimum version

The minimum version POD5 files are expected to be written is 0.3.31.